International Journal of Food and Nutritional Science | Volume 8 | Issue 1 | 2020-12-21

Antioxidant, Antimicrobial Activities and HPLC Quantitative Analysis of Some Sudanese Medicinal Plants

Abdalla Ahmed Elbashir

Abstract

The objective of this study was to evaluate the antimicrobial and antioxidant activities and to determine some of bioactive components of ethanolic extract of Adansonia digitata fruit pulp, Hibiscus sabdariffa flower, Hyphaene the baica fruit pulp and Ziziphus spina Christi fruit pulp. The ethanolic extracts of the plants were tested against four bacterial strains (Escherichia coli, Pseudomonasaeruginosa, Bacillus subtilis and Staphylococcus aureus) and two fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. Different plants extracts showed variable activity against all the tested microorganisms. The plant extracts were evaluated for their antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assays. The highest result of antioxidant activity by DPPH and ABTS assays was observed in Adansonia digitata fruit pulp extract (77.4%) with IC50 of 0.273 g mL-1 and Hyphaene the baica fruit pulp extract (79.02%) with IC50 value of 0.0808 g mL-1. The identification and quantification of ascorbic acid, gallic acid and quercetin in the samples ethanolic extracts was performed by a HPLC-PDA method. These compounds have been detected in all the tested extracts.

Keywords: HPLC-PDA, Antioxidant Activity, Antimicrobial Activity, Sudanese medicinal plants, Bioactive compounds