Journal of Analytical, Bioanalytical and Separation Techniques | Volume 3 | Issue 1 | 2018-08-02

SIMULTANEOUS ESTIMATION OF MINOXIDIL AND FINASTERIDE BY RP-HPLC IN PRESENCE OF SOY LECITHIN EXCIPIENT IN THE LOTION DOSAGE FORM

PONNILAVARASAN ILANKOVAN

Abstract

A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Minoxidil and Finasteride from bulk and liquid formulations in the presence of soy lecithin excipient. Chromatographic separation was achieved isocratically on a Phenomenex C18 column (250 4.6 mm, 5 particle size) using a mobile phase, Phosphate buffer: acetonitrile: methanol (50:50 v/v) (adjusted to pH 5.8 with NaOH) in the ratio of 30:70. The flow rate was 1.2 ml/min and effluent was detected at 211nm. The retention time of Minoxidil and Finasteride were 3.16, and 8.23 min respectively. Linearity was observed in the concentration range of 50 to 250 g/ml, 1 to 5 g/ml for Minoxidil and finasteride with correlation coefficient 0.999 for both the drugs. Percent recoveries obtained for both the drugs were 99.275-99.72 and 98.54-99.3% w/w. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Minoxidil and Finasteride from their combined dosage form. By this method, lecithin excipient having strong absorbance in the UV region minimized in the formulation as the excipient used for permeation enhancer is which has strong absorbance in the UV region it is minimized by this extraction method methanol and the mobile phase used for the RP-HPLC method.