Journal of Anesthesia and Surgery | 2015-05-08

Effect of Propofol on Expression of PKC mRNA during Pulmonary Ischemia-reperfusion Injury

Dong Song

Abstract

The aim was to study the effect of propofol on the expression of protein kinase C(PKC)mRNA during pulmonary ischemia-reperfusion injury (PIRI) in rabbits.Twenty-seven experimental rabbits were randomly divided into three groups(n=9):sham-operated group(Sham), PIR group(I/R) and PIR+propofol group(PPF). Changes of several parameters, including malondialdehyde (MDA) ,superoxide dismutase (SOD), lung wet/dry weight (W/D) and the index of quantitative assessment (IQA) of histologic lung injury were measured at 60 minutes after reperfusion. Meanwhile the location and expression of PKC mRNA were observed. Lung tissues were examined under the optical microscope and the electron microscope. As compared with group I/R, PKC mRNA notably expressed in intima and extima of small pulmonary artery as well as thin-wall vessels in PPF group.The average optical density values of PKC-α、δ and θ mRNA in small pulmonary veins in group PPF had significance (all P<0.01).SOD increased ,while MDA,W/D and IQA decreased at 60 minutes after reperfusion in lung tissue (P<0.01 and P<0.05). Abnormal changes of the lung tissues on morphologly were lessen markedly in group PPF.In conclusion,we demonstrated that propofol possessed significantly protective effects on PIRI in rabbits by activating PKC-α、δ and θ mRNA expression in lung tissue,raising NO level,reducing OFR level and decreasing lipid peroxidation.

Keywords: Ischemia-reperfusion injury, Lung, protein kinase C, Oxygen free radicals, Nitric oxide, Propofol